Enzymatic Reaction of alpha-Amylase With Starch and Its Monitoring by an Online Capillary Electrophoresis Method

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F17%3A10371612" target="_blank" >RIV/00216208:11310/17:10371612 - isvavai.cz</a>
Result on the web
<a href="http://web.natur.cuni.cz/analchem/isc/isc13.pdf" target="_blank" >http://web.natur.cuni.cz/analchem/isc/isc13.pdf</a>
DOI - Digital Object Identifier
—

Result language
angličtina
Original language name
Enzymatic Reaction of alpha-Amylase With Starch and Its Monitoring by an Online Capillary Electrophoresis Method
Original language description
Pancreatic α-amylase plays a crucial role in polysaccharide metabolism located in small intestine. Thus, inhibition of this enzyme is important for patients suffering from diabetes mellitus. In this study, we developed an online capillary electrophoresis method to monitor time course of enzymatic reaction of α-amylase with starch. Since the enzymatic reaction takes place in a capillary and the introduction process is automated by introducing zones of substrate and enzyme separately, fast screening of possible inhibitors is enabled within 6 minutes. Moreover, consumption of enzyme and substrate is decreased significantly.
Czech name
—
Czech description
—

Project
—
Continuities
S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
Proceedings of the 13th International Students Conference Modern Analytical Chemistry
ISBN
978-80-7444-052-6
ISSN
—
e-ISSN
—
Number of pages
6
Pages from-to
51-56
Publisher name
Univerzita Karlova, Přírodovědecká fakulta
Place of publication
Praha
Event location
Prague
Event date
Sep 21, 2017
Type of event by nationality
EUR - Evropská akce
UT code for WoS article
000435216500010

Similar results(10)