Study of Enzymatic Reaction by EMMA in a Partially Filled Capillary and Indirect or Direct Detection

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F04%3A00009907" target="_blank" >RIV/00216224:14310/04:00009907 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Study of Enzymatic Reaction by EMMA in a Partially Filled Capillary and Indirect or Direct Detection

Original language description

This report describes the application of electrophoretically mediated microanalysis (EMMA) in combination with a partial filling technique and indirect or direct detection for the study of enzymes dealing with the high mobile inorganic or organic anionsas substrates or products. In this set-up the part of the capillary is filled with the buffer best for the enzymatic reaction whereas the rest of the capillary with the background electrolyte optimal for separation of substrates and products. In the caseof haloalkane dehalogenase, which was chosen as a model enzyme, the enzymatic reaction was performed in 20 mM glycine buffer (pH 8.6). Because of wide substrate specifity of the enzyme, it utilizes chlorinated as well as brominated substrates producingeither non-absorbing chloride, or absorbing bromide ions, two different background electrolytes and the detection approaches were applied. A 10 mM chromate - 0.1 mM cetyltrimethylammonium bromide (pH 9.2) was used in combination with indi

Czech name

Studium enzymových reakcí metodou EMMA v částečně plněné kapiláře s přímou nebo nepřímou detekcí.

Czech description

Jedná se o studium enzymových reakcí metodou EMMA v částečně plněné kapiláře s přímou nebo nepřímou detekcí.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2004

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Electrophoresis

ISSN

0173-0835

e-ISSN

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Volume of the periodical

25

Issue of the periodical within the volume

2

Country of publishing house

US - UNITED STATES

Number of pages

9

Pages from-to

290-298

UT code for WoS article

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EID of the result in the Scopus database

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