Electrophoretically mediated microanalysis with partial filling technique and indirect or direct detection as a tool for inhibition studies of enzymatic reactions

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F04%3A00009987" target="_blank" >RIV/00216224:14310/04:00009987 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Electrophoretically mediated microanalysis with partial filling technique and indirect or direct detection as a tool for inhibition studies of enzymatic reactions

Original language description

The inhibition study of the model enzyme haloalkane dehalogenase from Sphingomonas paucimobilis was performed by a combination of the electrophoretically mediated microanalysis with a partial filling technique and indirect or direct detection. In this set-up, the part of capillary is filled with the buffer suitable for the enzymatic reaction 20 mM glycine buffer (pH 8.6) whereas, the rest of the capillary is filled with the background electrolyte optimal for separation of substrates and products. Two different background electrolytes and corresponding detection approaches were used to show the versatility of the developed method. The inhibition effect of 1,2-dichloroethane on the dehalogenation of brominated substrate 1-bromobutane was studied by meansof 10 mM chromate - 0.1 mM cetyltrimethylammonium bromide (pH 9.2) in combination with an indirect detection or 0.1 M beta-alanine - hydrochloric acid (pH 3.5) in combination with a direct detection. The method was used not only to estim

Czech name

Studium inhibice enzymů kombinací metody EMMA s technikou částečného plnění kapiláry a přímou či nepřímou detekcí.

Czech description

Jedna se o studium inhibice enzymů kombinací metody EMMA s technikou částečného plnění kapiláry a přímou či nepřímou detekcí.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2004

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Electrophoresis

ISSN

0173-0835

e-ISSN

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Volume of the periodical

24

Issue of the periodical within the volume

7-8

Country of publishing house

US - UNITED STATES

Number of pages

6

Pages from-to

1028

UT code for WoS article

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EID of the result in the Scopus database

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