Use high-performance liquid chromatography for determination of saccharides after the enzymatic hydrolysis of waste bread

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F14%3APU112104" target="_blank" >RIV/00216305:26310/14:PU112104 - isvavai.cz</a>
Result on the web
<a href="http://www.ce-ce.org/CECE2014/CECE%202014%20proceedings_full.pdf" target="_blank" >http://www.ce-ce.org/CECE2014/CECE%202014%20proceedings_full.pdf</a>
DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Use high-performance liquid chromatography for determination of saccharides after the enzymatic hydrolysis of waste bread
Original language description
The main topic of our study was the effect of pH and temperature on the efficiency of enzymatic hydrolysis. For study of enzymatic hydrolysis were used spectrophotometric and HPLC methods. Optimal value of pH and temperature of liquefaction was determined spectrophotometrically by Somogyi-Nelson method. Final yields of glucose from hydrolysis and efficiency of saccharification were determined by HPLC method. As substrate for enzymatic hydrolysis was used waste bread. It was pre-treated by grinding into small particles and then it was made to form 15% w/v suspension. For the hydrolysis have been used two enzymes. For liquefaction of the substrate we used α amylase, and for saccharification we used glucoamylase. It was found that optimal conditions for α amylase were pH 6 and temperature 80 °C. Under these conditions the yield of reducing sugars was 67.08 g∙L-1 (calculated to maltose). Optimal conditions for glucoamylase were pH 4.2 and temperature 60 °C. Obtained yield of glucose was 70.28 g∙L 1.
Czech name
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Czech description
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Type
O - Miscellaneous
CEP classification
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OECD FORD branch
20902 - Bioprocessing technologies (industrial processes relying on biological agents to drive the process) biocatalysis, fermentation

Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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