Optimization of Enzymatic Hydrolysis of Waste Bread before Fermentation
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F17%3APU122903" target="_blank" >RIV/00216305:26310/17:PU122903 - isvavai.cz</a>
Result on the web
<a href="https://acta.mendelu.cz/65/1/0035/" target="_blank" >https://acta.mendelu.cz/65/1/0035/</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.11118/actaun201765010035" target="_blank" >10.11118/actaun201765010035</a>
Alternative languages
Result language
angličtina
Original language name
Optimization of Enzymatic Hydrolysis of Waste Bread before Fermentation
Original language description
Finding of optimal hydrolysis conditions is important for increasing the yield of saccharides. The higher yield of saccharides is usable for increase of the following fermentation effectivity. In this study optimal conditions (pH and temperature) for amylolytic enzymes were searched. As raw material was used waste bread. Two analytical methods for analysis were used. Efficiency and process of hydrolysis was analysed spectrophotometrically by Somogyi-Nelson method. Final yields of glucose were analysed by HPLC. As raw material was used waste bread from local cafe. Waste bread was pretreated by grinding into small particles. Hydrolysis was performed in 100 mL of 15 % (w/v) waste bread particles in the form of water suspension. Waste bread was hydrolysed by two commercial enzymes. For the liquefaction was used α‑amylase (BAN 240 L). The saccharification was performed by glucoamylase (AMG 300 L). Optimal conditions for α‑amylase (pH 6; 80 °C) were found. The yield of total sugars was 67.08 g∙L-1 (calculated to maltose). As optimal conditions for glucoamylase (pH 4.2; 60 °C) were found. Amount of glucose was 70.28 g∙L1. The time of waste bread liquefaction was 180 minutes. The time of saccharification was 90 minutes. The results were presented at the conference CECE Junior 2014.
Czech name
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Czech description
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Classification
Type
J<sub>SC</sub> - Article in a specialist periodical, which is included in the SCOPUS database
CEP classification
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OECD FORD branch
20801 - Environmental biotechnology
Result continuities
Project
<a href="/en/project/LO1211" target="_blank" >LO1211: Materials Research Centre at FCH BUT- Sustainability and Development</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis
ISSN
1211-8516
e-ISSN
2464-8310
Volume of the periodical
65
Issue of the periodical within the volume
1
Country of publishing house
CZ - CZECH REPUBLIC
Number of pages
6
Pages from-to
35-40
UT code for WoS article
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EID of the result in the Scopus database
2-s2.0-85015947299