Offline and online capillary electrophoresis enzyme assays of beta-N-acetylhexosaminidase

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F13%3A10133390" target="_blank" >RIV/00216208:11310/13:10133390 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1007/s00216-012-6607-1" target="_blank" >http://dx.doi.org/10.1007/s00216-012-6607-1</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00216-012-6607-1" target="_blank" >10.1007/s00216-012-6607-1</a>

Result language

angličtina

Original language name

Offline and online capillary electrophoresis enzyme assays of beta-N-acetylhexosaminidase

Original language description

Enzyme assays of beta-N-acetylhexosaminidase from Aspergillus oryzae using capillary electrophoresis in the offline and online setup have been developed. The pH value and concentration of the borate-based background electrolyte were optimized in order toachieve baseline separation of N,N',N"-triacetylchitotriose, N,N'-diacetylchitobiose, and N-acetyl-D-glucosamine. The optimized method using 25 mM tetraborate buffer, pH 10.0, was evaluated in terms of repeatability, limits of detection, quantification,and linearity. The method was successfully applied to the offline enzyme assay of beta-N-acetylhexosaminidase, which was demonstrated by monitoring the hydrolysis of N,N',N"- triacetylchitotriose. The presented method was also utilized to study the pH dependence of enzyme activity. An online assay with N,N'-diacetylchitobiose as a substrate was developed using the Transverse Diffusion of Laminar Flow Profiles model to optimize the injection sequence and incapillary mixing of substrate a

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

<a href="/en/project/GBP206%2F12%2FG151" target="_blank" >GBP206/12/G151: Center of novel approaches to bioanalysis and molecular diagnostics</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2013

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Analytical and Bioanalytical Chemistry

ISSN

1618-2642

e-ISSN

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Volume of the periodical

405

Issue of the periodical within the volume

8

Country of publishing house

DE - GERMANY

Number of pages

10

Pages from-to

2425-2434

UT code for WoS article

000315464800003

EID of the result in the Scopus database

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