Immobilized-enzyme reactors integrated with capillary electrophoresis for pharmaceutical research

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F18%3A00100729" target="_blank" >RIV/00216224:14310/18:00100729 - isvavai.cz</a>
Result on the web
<a href="http://onlinelibrary.wiley.com/doi/10.1002/jssc.201700905/abstract?campaign=wolacceptedarticle" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1002/jssc.201700905/abstract?campaign=wolacceptedarticle</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/jssc.201700905" target="_blank" >10.1002/jssc.201700905</a>

Result language
angličtina
Original language name
Immobilized-enzyme reactors integrated with capillary electrophoresis for pharmaceutical research
Original language description
Enzymes play an essential role in many aspects of pharmaceutical research as drug targets, drug metabolizers, enzyme drugs and more. In this specific field, enzyme assays are required to meet a number of specific requirements, such as low cost, easy automation and high reliability. The integration of an immobilized-enzyme reactor to capillary electrophoresis represents a unique approach to fulfilling these criteria by combining the benefits of enzyme immobilization, that is, increased stability and repeated use, as well as the minute sample consumption, short analysis time and efficient analysis provided by capillary electrophoresis. In this review, we summarize, analyse and discuss published works where pharmaceutically relevant enzymes were used to prepare capillary-electrophoresis-integrated immobilized-enzyme reactors in an online manner. The presented assays are divided into three distinct groups based on the drug–enzyme relationship. The first, more extensively studied group employs enzymes which are considered to be therapeutic targets, the second group of assays presents tools to assess drug metabolism and the third group assesses enzyme drugs. Furthermore, we examine various methods of enzyme immobilization and their implications for assay properties.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10600 - Biological sciences

Project
<a href="/en/project/GA16-06106S" target="_blank" >GA16-06106S: Capillary electrophoresis based high-throughput system for beta-secretase inhibitor screening as a therapeutic target of Alzheimer's disease</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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