TOWARDS CYTOCHROME P450 IMER FOR KINETICS AND INHIBITION STUDIES USING CAPILLARY ELECTROPHORESIS IN ONLINE CONFIGURATION
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F14%3A00074138" target="_blank" >RIV/00216224:14740/14:00074138 - isvavai.cz</a>
Result on the web
—
DOI - Digital Object Identifier
—
Alternative languages
Result language
angličtina
Original language name
TOWARDS CYTOCHROME P450 IMER FOR KINETICS AND INHIBITION STUDIES USING CAPILLARY ELECTROPHORESIS IN ONLINE CONFIGURATION
Original language description
The enzyme superfamily of cytochromes P450 (CYP) plays a key role in xenobiotic biotransformation and thusly in drug metabolism. Therefore assessment of the interaction between candidate drugs and CYP isoforms is an integral part of preclinical stage ofthe drug development process, where high throughput and cost effectiveness is a priority. Capillary electrophoresis (CE) offers a number of features which are suitable for these purposes, those being the low sample consumption, short analysis time and parallel analyses using multicapillary systems, what more integration of immobilized enzyme microreactor (IMER) into the separation capillary enables full automation and repeated use of the enzyme. A new online method for inhibition studies based on cytochrome P450 2C9 isoform IMER integrated into CE system is thus presented.
Czech name
—
Czech description
—
Classification
Type
D - Article in proceedings
CEP classification
CE - Biochemistry
OECD FORD branch
—
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach
Others
Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
11 th International Interdisciplinary Meeting on Bioanalysis CECE 2014
ISBN
9788090495920
ISSN
—
e-ISSN
—
Number of pages
2
Pages from-to
131-132
Publisher name
Institute of Analytical Chemistry AS CR, v. v. i., Brno, Česká republika
Place of publication
Brno
Event location
Brno
Event date
Oct 20, 2014
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
—