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Application of On-line Capillary Electrophoresis Coupled to Mass Spectrometry for Identification of Cytochrome P450 2C9 Isoform Metabolism Products

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F12%3A00057805" target="_blank" >RIV/00216224:14310/12:00057805 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Application of On-line Capillary Electrophoresis Coupled to Mass Spectrometry for Identification of Cytochrome P450 2C9 Isoform Metabolism Products

  • Original language description

    Drug biotransformation mediated by cytochrome P450 enzymes (CYP) is a pivotal factor in the early developmental stages of new drugs. Metabolism appraisal of every hit thus constitutes the inseparable part of ADME/tox screenings. Capillary electrophoresis(CE) represents a promising technique in the field of enzyme assays due to miniscule sample consumption and high-throughput by automation. Moreover, CE coupled to mass spectrometry (MS) combines the advantages of both techniques so that CYP metabolism products can be identified on the bases of migration time, molecular weight and fragmentation. A new CE-MS method enabling identification of reaction products provided by clinically important CYP2C9 isoform is thus introduced.

  • Czech name

  • Czech description

Classification

  • Type

    D - Article in proceedings

  • CEP classification

    CE - Biochemistry

  • OECD FORD branch

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2012

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Article name in the collection

    9th International Interdisciplinary Meeting on Bioanalysis, CECE 2012

  • ISBN

    9788090495913

  • ISSN

  • e-ISSN

  • Number of pages

    4

  • Pages from-to

    368-371

  • Publisher name

    IACH AV ČR Brno

  • Place of publication

    Brno

  • Event location

    Brno

  • Event date

    Nov 1, 2012

  • Type of event by nationality

    WRD - Celosvětová akce

  • UT code for WoS article