Capillary electrophoresis-integrated immobilized enzyme reactor for kinetic and inhibition assays of beta-secretase as the Alzheimer's disease drug target
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F19%3A00107212" target="_blank" >RIV/00216224:14310/19:00107212 - isvavai.cz</a>
Result on the web
<a href="https://onlinelibrary.wiley.com/doi/full/10.1002/jssc.201800947" target="_blank" >https://onlinelibrary.wiley.com/doi/full/10.1002/jssc.201800947</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/jssc.201800947" target="_blank" >10.1002/jssc.201800947</a>
Alternative languages
Result language
angličtina
Original language name
Capillary electrophoresis-integrated immobilized enzyme reactor for kinetic and inhibition assays of beta-secretase as the Alzheimer's disease drug target
Original language description
Capillary electrophoresis-integrated immobilized enzyme reactors are becoming an increasingly popular alternative for enzyme kinetic and inhibition assays thanks to their unique set of features including cost effectiveness, repeated use of the enzyme, minuscule sample consumption, rapid analysis time and easy automation. In this work we present the development and application of a CE-integrated immobilized enzyme reactor based on magnetic particles for kinetic and inhibition studies of beta secretase, a key enzyme in the development of Alzheimer’s disease and a promising drug target. We document the optimization of the immobilization procedure, characterization of immobilized beta secretase, optimization of a mutually compatible incubation protocol and separation method as well as the production of the CE-integrated immobilized enzyme reactor. The applicability of the CE-integrated immobilized enzyme reactor was demonstrated by kinetic assay with an unlabelled substrate and by inhibition assays using three structurally different reference inhibitors. The resulting kinetic and inhibition parameters clearly support the applicability of the herein presented method as well as document the fundamental phenomena which need to be taken in account when comparing the results to other methods.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
<a href="/en/project/GA16-06106S" target="_blank" >GA16-06106S: Capillary electrophoresis based high-throughput system for beta-secretase inhibitor screening as a therapeutic target of Alzheimer's disease</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Separation Science
ISSN
1615-9306
e-ISSN
1615-9314
Volume of the periodical
42
Issue of the periodical within the volume
5
Country of publishing house
DE - GERMANY
Number of pages
10
Pages from-to
1067-1076
UT code for WoS article
000461674900013
EID of the result in the Scopus database
2-s2.0-85061060350