Induced Maturation of Human Immunodeficiency Virus

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F14%3A10286918" target="_blank" >RIV/00216208:11310/14:10286918 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1128/JVI.02271-14" target="_blank" >http://dx.doi.org/10.1128/JVI.02271-14</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1128/JVI.02271-14" target="_blank" >10.1128/JVI.02271-14</a>

Result language

angličtina

Original language name

Induced Maturation of Human Immunodeficiency Virus

Original language description

HIV-1 assembles at the plasma membrane of virus-producing cells as an immature, noninfectious particle. Processing of the Gag and Gag-Pol polyproteins by the viral protease (PR) activates the viral enzymes and results in dramatic structural rearrangements within the virion-termed maturation-that are a prerequisite for infectivity. Despite its fundamental importance for viral replication, little is currently known about the regulation of proteolysis and about the dynamics and structural intermediates ofmaturation. This is due mainly to the fact that HIV-1 release and maturation occur asynchronously both at the level of individual cells and at the level of particle release from a single cell. Here, we report a method to synchronize HIV-1 proteolysis invitro based on protease inhibitor (PI) washout from purified immature virions, thereby temporally uncoupling virus assembly and maturation. Drug washout resulted in the induction of proteolysis with cleavage efficiencies correlating with

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EE - Microbiology, virology

OECD FORD branch

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Project

<a href="/en/project/GBP208%2F12%2FG016" target="_blank" >GBP208/12/G016: Controlling structure and function of biomolecules at the molecular scale: theory meets experiment</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Virology

ISSN

0022-538X

e-ISSN

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Volume of the periodical

88

Issue of the periodical within the volume

23

Country of publishing house

US - UNITED STATES

Number of pages

10

Pages from-to

13722-13731

UT code for WoS article

000344812800017

EID of the result in the Scopus database

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