Protein and Site Specificity of Fucosylation in Liver-Secreted Glycoproteins

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F14%3A10288337" target="_blank" >RIV/00216208:11310/14:10288337 - isvavai.cz</a>

Alternative codes found

RIV/61388971:_____/14:00440676

Result on the web

<a href="http://dx.doi.org/10.1021/pr5005482" target="_blank" >http://dx.doi.org/10.1021/pr5005482</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1021/pr5005482" target="_blank" >10.1021/pr5005482</a>

Result language

angličtina

Original language name

Protein and Site Specificity of Fucosylation in Liver-Secreted Glycoproteins

Original language description

Chronic liver diseases are a serious health problem worldwide. One of the frequently reported glycan alterations in liver disease is aberrant fucosylation, which was suggested as a marker for noninvasive serologic monitoring. We present a case study thatcompares site specific glycoforms of four proteins including haptoglobin, complement factor H, kininogen-1, and hemopexin isolated from the same patient. Our exoglycosidase-assisted LC-MS/MS analysis confirms the high degree of fucosylation of some of the proteins but shows that microheterogeneity is protein- and site-specific. MSn analysis of permethylated detached glycans confirms the presence of LeY glycoforms on haptoglobin, which cannot be detected in hemopexin or complement factor H; all three proteins carry Lewis and H epitopes. Core fucosylation is detectable in only trace amounts in haptoglobin but with confidence on hemopexin and complement factor H, where core fucosylation of the bi-antennary glycans on select glycopeptides

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Proteome Research

ISSN

1535-3893

e-ISSN

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Volume of the periodical

13

Issue of the periodical within the volume

12

Country of publishing house

US - UNITED STATES

Number of pages

9

Pages from-to

5561-5569

UT code for WoS article

000346039400024

EID of the result in the Scopus database

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