Quantification of Fucosylated Hemopexin and Complement Factor H in Plasma of Patients with Liver Disease

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F14%3A10288480" target="_blank" >RIV/00216208:11310/14:10288480 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1021/ac502727s" target="_blank" >http://dx.doi.org/10.1021/ac502727s</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1021/ac502727s" target="_blank" >10.1021/ac502727s</a>

Result language

angličtina

Original language name

Quantification of Fucosylated Hemopexin and Complement Factor H in Plasma of Patients with Liver Disease

Original language description

Enhanced fucosylation has been suggested as a marker for serologic monitoring of liver disease and hepatocellular carcinoma (HCC). We present a workflow for quantitative site-specific analysis of fucosylation and apply it to a comparison of hemopexin (HPX) and complement factor H (CFH), two liver-secreted glycoproteins, in healthy individuals and patients with liver cirrhosis and HCC. Label-free LC-MS quantification of glycopeptides derived from these purified glycoproteins was performed on pooled samples (2 pools/group, 5 samples/pool) and complemented by glycosidase assisted analysis using sialidase and endoglycosidase F2/F3, respectively, to improve resolution of glycoforms. Our analysis, presented as relative abundance of individual fucosylated glycoforms normalized to the level of their nonfucosylated counterparts, revealed a consistent increase in fucosylation in liver disease with significant site- and protein-specific differences. We have observed the highest microheterogeneity

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Analytical Chemistry

ISSN

0003-2700

e-ISSN

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Volume of the periodical

86

Issue of the periodical within the volume

21

Country of publishing house

US - UNITED STATES

Number of pages

8

Pages from-to

10716-10723

UT code for WoS article

000344510200032

EID of the result in the Scopus database

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