Study of photodynamic effects on NIH 3T3 cell line and bacteria
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F14%3A10288509" target="_blank" >RIV/00216208:11310/14:10288509 - isvavai.cz</a>
Alternative codes found
RIV/61989592:15110/14:33152280
Result on the web
<a href="http://dx.doi.org/10.5507/bp.2012.057" target="_blank" >http://dx.doi.org/10.5507/bp.2012.057</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.5507/bp.2012.057" target="_blank" >10.5507/bp.2012.057</a>
Alternative languages
Result language
angličtina
Original language name
Study of photodynamic effects on NIH 3T3 cell line and bacteria
Original language description
Background. Bacterial resistance to antibiotics is a constantly growing challenge. Photodynamic therapy (PDT) offers a new approach to the treatment of bacterial and viral diseases. The aim of this study was to compare the efficacy of photosensitizers used in PDT applied to cell lines and bacterial strains. Methods. We tested the cytotoxicity and phototoxicity of 3 photosensitizers: TPPS 4, ZnTPPS 4 and TMPyP applied to the NIH3T3 cell line using two established methods for measuring ROS production and,MTT viability assay. Bacterial viability was determined spectrophotometrically over 24 h following PDT. Results. The most efficient photosensitiser was TMPyP as it reduced the viability of the NIH3T3 cell line by more than 85%. In general, the photosensitisers were more phototoxic to the two Gram-positive bacterial strains, Enterococcus faecalis and Staphylococcus aureus. The viability of E. faecalis was reduced to 78 % by a dose radiation 0.5 J/cm2 and concentration of TMPyP 1.562 mol/
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
BO - Biophysics
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Biomedical Papers
ISSN
1213-8118
e-ISSN
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Volume of the periodical
158
Issue of the periodical within the volume
2
Country of publishing house
CZ - CZECH REPUBLIC
Number of pages
7
Pages from-to
201-207
UT code for WoS article
000338628200007
EID of the result in the Scopus database
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