Comparison of commonly used primer sets for evaluating arbuscular mycorrhizal fungal communities: Is there a universal solution?

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F14%3A10293606" target="_blank" >RIV/00216208:11310/14:10293606 - isvavai.cz</a>
Alternative codes found
RIV/60077344:_____/14:00428816 RIV/67985939:_____/14:00429493
Result on the web
<a href="http://dx.doi.org/10.1016/j.soilbio.2013.08.027" target="_blank" >http://dx.doi.org/10.1016/j.soilbio.2013.08.027</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.soilbio.2013.08.027" target="_blank" >10.1016/j.soilbio.2013.08.027</a>

Result language
angličtina
Original language name
Comparison of commonly used primer sets for evaluating arbuscular mycorrhizal fungal communities: Is there a universal solution?
Original language description
Different primer systems have been developed to characterize arbuscular mycorrhizal fungal (AMF) communities; however, a direct comparison of their specificity, potential to describe diversity and representation of different phylogenetic lineages is lacking. Using seven root samples, we compared four routinely used AMF-specific primer systems for nuclear ribosomal DNA covering i) the partial small subunit (SSU), ii) the partial large subunit (LSU), iii) the partial SSU and internal transcribed spacer (ITS; "Redecker") and iv) the partial SSU ITS partial LSU region ("Kruger"). In addition, a new primer combination v) covering the ITS2 region (ITS2) was included in the comparison. The "Kruger" primers tended to yield the highest AMF diversity and showeda significantly higher Shannon diversity index than the SSU primers. We found a strong bias towards the Glomeraceae in the LSU and SSU primer systems and differences in the composition of AMF communities based on the "Redecker" primer sys
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EF - Botany
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
S - Specificky vyzkum na vysokych skolach

Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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