Microtubule Nucleation in Mouse Bone Marrow-Derived Mast Cells Is Regulated by the Concerted Action of GIT1/?PIX Proteins and Calcium

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F15%3A10293952" target="_blank" >RIV/00216208:11310/15:10293952 - isvavai.cz</a>

Alternative codes found

RIV/68378050:_____/15:00455873

Result on the web

<a href="http://dx.doi.org/10.4049/jimmunol.1402459" target="_blank" >http://dx.doi.org/10.4049/jimmunol.1402459</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.4049/jimmunol.1402459" target="_blank" >10.4049/jimmunol.1402459</a>

Result language

angličtina

Original language name

Microtubule Nucleation in Mouse Bone Marrow-Derived Mast Cells Is Regulated by the Concerted Action of GIT1/?PIX Proteins and Calcium

Original language description

Ag-mediated activation of mast cells initiates signaling events leading to Ca(2+) response, release of allergic mediators from cytoplasmic granules, and synthesis of cytokines and chemokines. Although microtubule rearrangement during activation has beendescribed, the molecular mechanisms that control their remodeling are largely unknown. Microtubule nucleation is mediated by complexes that are formed by ?-tubulin and ?-tubulin complex proteins. In this study, we report that, in bone marrow-derived mastcells (BMMCs), ?-tubulin interacts with p21-activated kinase interacting exchange factor ? (?PIX) and G protein-coupled receptor kinase-interacting protein (GIT)1. Microtubule regrowth experiments showed that the depletion of ?PIX in BMMCs stimulated microtubule nucleation, whereas depletion of GIT1 led to the inhibition of nucleation compared with control cells. Phenotypic rescue experiments confirmed that ?PIX and GIT1 represent negative and positive regulators of microtubule nucleati

Czech name

—

Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EC - Immunology

OECD FORD branch

—

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

S - Specificky vyzkum na vysokych skolach

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Immunology

ISSN

0022-1767

e-ISSN

—

Volume of the periodical

194

Issue of the periodical within the volume

9

Country of publishing house

US - UNITED STATES

Number of pages

13

Pages from-to

4099-4111

UT code for WoS article

000353727400009

EID of the result in the Scopus database

2-s2.0-84928484964