Methylation analysis of histone H4K12ac-associated promoters in sperm of healthy donors and subfertile patients

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F15%3A10294696" target="_blank" >RIV/00216208:11310/15:10294696 - isvavai.cz</a>
Alternative codes found
RIV/86652036:_____/15:00443675
Result on the web
<a href="http://dx.doi.org/10.1186/s13148-015-0058-4" target="_blank" >http://dx.doi.org/10.1186/s13148-015-0058-4</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1186/s13148-015-0058-4" target="_blank" >10.1186/s13148-015-0058-4</a>

Result language
angličtina
Original language name
Methylation analysis of histone H4K12ac-associated promoters in sperm of healthy donors and subfertile patients
Original language description
Histone to protamine exchange and the hyperacetylation of the remaining histones are hallmarks of spermiogenesis. Acetylation of histone H4 at lysine 12 (H4K12ac) was observed prior to full decondensation of sperm chromatin after fertilization suggestingan important role for the regulation of gene expression in early embryogenesis. Similarly, DNA methylation may contribute to gene silencing of several developmentally important genes. Following the identification of H4K12ac-binding promoters in sperm offertile and subfertile patients, we aimed to investigate whether the depletion of histone-binding is associated with aberrant DNA methylation in sperm of subfertile men. Furthermore, we monitored the transmission of H4K12ac, 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) from the paternal chromatin to the embryo applying mouse in vitro fertilization and immunofluorescence. We found 5% to 10% hypomethylation within CpG islands of selected promoters in the sperm of fertile dono
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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