Cholesterol modulates open probability and desensitization of NMDA receptors

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F15%3A10295002" target="_blank" >RIV/00216208:11310/15:10295002 - isvavai.cz</a>

Alternative codes found

RIV/67985823:_____/15:00446418 RIV/00216208:11130/15:10295002

Result on the web

<a href="http://dx.doi.org/10.1113/jphysiol.2014.288209" target="_blank" >http://dx.doi.org/10.1113/jphysiol.2014.288209</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1113/jphysiol.2014.288209" target="_blank" >10.1113/jphysiol.2014.288209</a>

Result language

angličtina

Original language name

Cholesterol modulates open probability and desensitization of NMDA receptors

Original language description

NMDA receptors (NMDARs) are glutamate-gated ion channels that mediate excitatory neurotransmission in the CNS. Although these receptors are in direct contact with plasma membrane, lipid-NMDAR interactions are little understood. In the present study, we aimed at characterizing the effect of cholesterol on the ionotropic glutamate receptors. Whole-cell current responses induced by fast application of NMDA in cultured rat cerebellar granule cells (CGCs) were almost abolished (reduced to 3%) and the relative degree of receptor desensitization was increased (by seven-fold) after acute cholesterol depletion by methyl--cyclodextrin. Both of these effects were fully reversible by cholesterol repletion. By contrast, the responses mediated by AMPA/kainate receptors were not affected by cholesterol depletion. Similar results were obtained in CGCs after chronic inhibition of cholesterol biosynthesis by simvastatin and acute enzymatic cholesterol degradation to 4-cholesten-3-one by cholesterol oxid

Czech name

—

Czech description

—

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FH - Neurology, neuro-surgery, nuero-sciences

OECD FORD branch

—

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Physiology

ISSN

0022-3751

e-ISSN

—

Volume of the periodical

593

Issue of the periodical within the volume

10

Country of publishing house

GB - UNITED KINGDOM

Number of pages

15

Pages from-to

2279-2293

UT code for WoS article

000354646100004

EID of the result in the Scopus database

2-s2.0-84929502927