Resolution of alpha/beta-amino acids by enantioselective penicillin G acylase from Achromobacter sp.

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F15%3A10297850" target="_blank" >RIV/00216208:11310/15:10297850 - isvavai.cz</a>
Alternative codes found
RIV/61388971:_____/15:00455568 RIV/00216224:14310/15:00085553
Result on the web
<a href="http://dx.doi.org/10.1016/j.molcatb.2015.09.008" target="_blank" >http://dx.doi.org/10.1016/j.molcatb.2015.09.008</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.molcatb.2015.09.008" target="_blank" >10.1016/j.molcatb.2015.09.008</a>

Result language
angličtina
Original language name
Resolution of alpha/beta-amino acids by enantioselective penicillin G acylase from Achromobacter sp.
Original language description
Penicillin G acylases (PGAs) are enantioselective enzymes catalyzing a hydrolysis of stable amide bond in a broad spectrum of substrates. Among them, derivatives of ?MINUS SIGN and ?-amino acids represent a class of compounds with high application potential. PGAEc from Escherichia coli and PGAA from Achromobacter sp. CCM 4824 were used to catalyze enantioselective hydrolyses of seven selected N-phenylacetylated ?/#X?S#?et?;-amino acid racemates. The PGAA showed higher stereoselectivity for three enantiomers of N-PhAc-?-homoleucine, N-PhAc-?-tert-leucine and N-PhAc-?-leucine. To study the mechanism of enantiodiscrimination on molecular level, we have constructed a homology model of PGAA that was used in molecular docking experiments with the same substrates. In-silico experiments successfully reproduced the data from experimental enzymatic resolutions confirming validity of employed modeling protocol. We employed this protocol to evaluate enantiopreference of PGAA towards seven new subs
Czech name
—
Czech description
—

Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
—

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Similar results(10)