Involvement of PKC epsilon in Cardioprotection Induced by Adaptation to Chronic Continuous Hypoxia

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F15%3A10297864" target="_blank" >RIV/00216208:11310/15:10297864 - isvavai.cz</a>

Alternative codes found

RIV/67985823:_____/15:00444821

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Involvement of PKC epsilon in Cardioprotection Induced by Adaptation to Chronic Continuous Hypoxia

Original language description

Continuous normobaric hypoxia (CNH) renders the heart more tolerant to acute ischemia/reperfusion injury. Protein kinase C (PKC) is an important component of the protective signaling pathway, but the contribution of individual PKC isoforms under different hypoxic conditions is poorly understood. The aim of this study was to analyze the expression of PKCe after the adaptation to CNH and to clarify its role in increased cardiac ischemic tolerance with the use of PKCe inhibitory peptide KP-1633. Adult maleWistar rats were exposed to CNH (10 % O-2, 3 weeks) or kept under normoxic conditions. The protein level of PKCe and its phosphorylated form was analyzed by Western blot in homogenate, cytosolic and particulate fractions; the expression of PKCe mRNA wasmeasured by RT-PCR. The effect of KP-1633 on cell viability and lactate dehydrogenase (LDH) release was analyzed after 25-min metabolic inhibition followed by 30-min reenergization in freshly isolated left ventricular myocytes. Adaptatio

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FA - Cardiovascular diseases including cardio-surgery

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Physiological Research

ISSN

0862-8408

e-ISSN

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Volume of the periodical

64

Issue of the periodical within the volume

2

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

11

Pages from-to

191-201

UT code for WoS article

000357409400006

EID of the result in the Scopus database

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