The pool of preactivated Lck in the initiation of T-cell signaling: a critical re-evaluation of the Lck standby model

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F15%3A10314282" target="_blank" >RIV/00216208:11310/15:10314282 - isvavai.cz</a>

Alternative codes found

RIV/68378050:_____/15:00455198

Result on the web

<a href="http://dx.doi.org/10.1038/icb.2014.100" target="_blank" >http://dx.doi.org/10.1038/icb.2014.100</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/icb.2014.100" target="_blank" >10.1038/icb.2014.100</a>

Result language

angličtina

Original language name

The pool of preactivated Lck in the initiation of T-cell signaling: a critical re-evaluation of the Lck standby model

Original language description

The initiation of T-cell receptor (TCR) signaling, based on the cobinding of TCR and CD4-Lck heterodimer to a peptide-major histocompatibility complex II on antigen presenting cells, represents a classical model of T-cell signaling. What is less clear however, is the mechanism which translates TCR engagement to the phosphorylation of immunoreceptor tyrosine-based activation motifs on CD3 chains and how this event is coupled to the delivery of Lck function. Recently proposed ' standby model of Lck' posits that resting T-cells contain an abundant pool of constitutively active Lck (pY394(Lck)) required for TCR triggering, and this amount, upon TCR engagement, remains constant. Here, we show that although maintenance of the limited pool of pY394(Lck) is necessary for the generation of TCR proximal signals in a time-restricted fashion, the total amount of this pool, similar to 2%, is much smaller than previously reported (similar to 40%). We provide evidence that this dramatic discrepancy i

Czech name

—

Czech description

—

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EC - Immunology

OECD FORD branch

—

Project

<a href="/en/project/GA310%2F09%2F2084" target="_blank" >GA310/09/2084: Characterization of the molecular machinery regulating the recruitment of signaling molecules to lipid rafts</a><br>

Continuities

S - Specificky vyzkum na vysokych skolach

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Immunology and Cell Biology

ISSN

0818-9641

e-ISSN

—

Volume of the periodical

93

Issue of the periodical within the volume

4

Country of publishing house

AU - AUSTRALIA

Number of pages

12

Pages from-to

384-395

UT code for WoS article

000353302900011

EID of the result in the Scopus database

2-s2.0-84928209119