Arp2/3 complex subunit ARPC2 binds to microtubules

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F15%3A10315431" target="_blank" >RIV/00216208:11310/15:10315431 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1016/j.plantsci.2015.10.001" target="_blank" >http://dx.doi.org/10.1016/j.plantsci.2015.10.001</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.plantsci.2015.10.001" target="_blank" >10.1016/j.plantsci.2015.10.001</a>

Result language

angličtina

Original language name

Arp2/3 complex subunit ARPC2 binds to microtubules

Original language description

Arp2/3 complex plays a fundamental role in the nucleation of actin filaments (AFs) in yeasts, plants, and animals. In plants, the aberrant shaping and elongation of several types of epidermal cells observed in Arp2/3 complex knockout plant mutants suggest the importance of Arp2/3-mediated actin nucleation for various morphogenetic processes. Here we show that ARPC2, a core Arp2/3 complex subunit, interacts with both actin filaments (AFs) and microtubules (MTs). Plant GFP-ARPC2 expressed in Nicotiana tabacum BY-2 cells, leaf epidermal cells of Nicotiana benthamiana and root epidermal cells of Arabidopsis thaliana decorated MTs. The interaction with MTs was demonstrated by pharmacological approach selectively interfering with either AFs or MTs dynamics as well as by the in vitro cosedimentation assays. A putative MT-binding domain of tobacco NtARPC2 protein was identified using the co-sedimentation of several truncated NtARPC2 proteins with MTs. Newly identified MT-binding ability of ARP

Czech name

—

Czech description

—

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

<a href="/en/project/LO1417" target="_blank" >LO1417: Centre of Experimental Plant Biology of CU</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Plant Science

ISSN

0168-9452

e-ISSN

—

Volume of the periodical

241

Issue of the periodical within the volume

December 2015

Country of publishing house

IE - IRELAND

Number of pages

13

Pages from-to

96-108

UT code for WoS article

000367487500010

EID of the result in the Scopus database

2-s2.0-84944452065