Nuclear actin filaments recruit cofilin and actin-related protein 3, and their formation is connected with a mitotic block

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F14%3A00434269" target="_blank" >RIV/68378050:_____/14:00434269 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1007/s00418-014-1243-9" target="_blank" >http://dx.doi.org/10.1007/s00418-014-1243-9</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00418-014-1243-9" target="_blank" >10.1007/s00418-014-1243-9</a>

Result language

angličtina

Original language name

Nuclear actin filaments recruit cofilin and actin-related protein 3, and their formation is connected with a mitotic block

Original language description

Although actin monomers polymerize into filaments in the cytoplasm, the form of actin in the nucleus remains elusive. We searched for the form and function of ?-actin fused to nuclear localization signal and to enhanced yellow fluorescent protein (EN-actin). Our results reveal that EN-actin is either dispersed in the nucleoplasm (homogenous EN-actin) or forms bundled filaments in the nucleus (EN-actin filaments). Formation of such filaments was not connected with increased EN-actin levels. Among numerous actin-binding proteins tested, only cofilin is recruited to the EN-actin filaments. Overexpression of EN-actin causes increase in the nuclear levels of actin-related protein 3 (Arp3). Although Arp3, a member of actin nucleation complex Arp2/3, is responsible for EN-actin filament nucleation and bundling, the way cofilin affects nuclear EN-actin filaments dynamics is not clear. While cells with homogenous EN-actin maintained unaffected mitosis during which EN-actin re-localizes to the p

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Histochemistry and Cell Biology

ISSN

0948-6143

e-ISSN

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Volume of the periodical

142

Issue of the periodical within the volume

2

Country of publishing house

DE - GERMANY

Number of pages

14

Pages from-to

139-152

UT code for WoS article

000339963300003

EID of the result in the Scopus database

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