Cardioprotective adaptation of rats to intermittent hypobaric hypoxia is accompanied by the increased association of hexokinase with mitochondria
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F15%3A10321268" target="_blank" >RIV/00216208:11310/15:10321268 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1152/japplphysiol.01035.2014" target="_blank" >http://dx.doi.org/10.1152/japplphysiol.01035.2014</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1152/japplphysiol.01035.2014" target="_blank" >10.1152/japplphysiol.01035.2014</a>
Alternative languages
Result language
angličtina
Original language name
Cardioprotective adaptation of rats to intermittent hypobaric hypoxia is accompanied by the increased association of hexokinase with mitochondria
Original language description
Chronic hypoxia increases the myocardial resistance to acute ischemia-reperfusion injury by affecting the mitochondrial redox balance. Hexokinase (HK) bears a high potential to suppress the excessive formation of reactive oxygen species because of its increased association with mitochondria, thereby inhibiting the membrane permeability transition pore opening and preventing cell death. The purpose of this study was to determine the effect of severe intermittent hypobaric hypoxia (7,000 m, 8 h/day, 5 wk)on the function and colocalization of HK isoforms with mitochondria in the left (LV) and right ventricles of rat myocardium. The real-time RT-PCR, Western blot, enzyme coupled assay, and quantitative immunofluorescence techniques were used. Our resultsshowed significantly elevated expression of HK isoforms (HK1 and HK2) in the hypoxic LV. In addition, intermittent hypoxia increased the total HK activity and the association of HK isoforms with mitochondria in both ventricles. These find
Czech name
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Czech description
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Classification
Type
O - Miscellaneous
CEP classification
FA - Cardiovascular diseases including cardio-surgery
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GP14-04301P" target="_blank" >GP14-04301P: The role of mitochondria-associated hexokinase2 and the PI3K/Akt signalling pathway in the adaptation of rat heart to chronic hypoxia</a><br>
Continuities
S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů