Simple electrochemical DNA biosensor for detection of DNA damage induced by hydroxyl radicals
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F15%3A10328322" target="_blank" >RIV/00216208:11310/15:10328322 - isvavai.cz</a>
Result on the web
<a href="https://web.natur.cuni.cz/analchem/isc-mac/" target="_blank" >https://web.natur.cuni.cz/analchem/isc-mac/</a>
DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Simple electrochemical DNA biosensor for detection of DNA damage induced by hydroxyl radicals
Original language description
A novel simple electrochemical DNA biosensor based on a glassy carbon electrode was prepared by adsorbing low molecular weight double-stranded DNA from salmon sperm onto the electrode surface as a biorecognition layer. The biosensor was used for detection of DNA damage by hydroxyl radicals. Hydroxyl radicals are well-known reactive oxygen species inducing the oxidative stress in DNA. They can attack all the molecules, including DNA, and play a major role in the DNA oxidative damage. They were generated for our purposes electrochemically on the surface of a boron-doped diamond film electrode. A complex characterization of the specific damaging event was then obtained using a combination of several electrochemical detection techniques: cyclic voltammetry, square-wave voltammetry, and electrochemical impedance spectroscopy.
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GBP206%2F12%2FG151" target="_blank" >GBP206/12/G151: Center of novel approaches to bioanalysis and molecular diagnostics</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
PROCEEDINGS OF THE 11TH INTERNATIONAL STUDENTS CONFERENCE "MODERN ANALYTICAL CHEMISTRY"
ISBN
978-80-7444-036-6
ISSN
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e-ISSN
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Number of pages
4
Pages from-to
19-22
Publisher name
CHARLES UNIV, FAC SCIENCE
Place of publication
PRAGUE 2
Event location
Prague
Event date
Sep 22, 2015
Type of event by nationality
EUR - Evropská akce
UT code for WoS article
000383853900004