New strategy in electrochemical investigation of DNA damage demonstrated on genotoxic derivatives of fluorene
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F22%3A10445189" target="_blank" >RIV/00216208:11310/22:10445189 - isvavai.cz</a>
Result on the web
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=6wZuh9B4HQ" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=6wZuh9B4HQ</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.jelechem.2022.116430" target="_blank" >10.1016/j.jelechem.2022.116430</a>
Alternative languages
Result language
angličtina
Original language name
New strategy in electrochemical investigation of DNA damage demonstrated on genotoxic derivatives of fluorene
Original language description
In this work, a simple and fast electrochemical DNA biosensor based on a glassy carbon electrode (GCE) was prepared by adsorbing double-stranded DNA (dsDNA) from salmon sperm as a biorecognition layer onto the GCE surface. The dsDNA/GCE biosensor was characterized using modern imaging techniques. The prepared dsDNA/GCE biosensor was used for detection of dsDNA damage by 2-aminofluorene (2-AF), its metabolite 2-acetylaminofluorene (2-AAF), and 2,7-diaminofluorene (2,7-DAF). The electrochemical investigation of the interaction between the selected analytes and dsDNA was conducted using a combination of several electrochemical detection techniques (representing a comprehensive electrochemical biosensing approach allowing an overall assessment of dsDNA damage, even when the intrinsic voltammetric signals of the studied analytes overlap with the dsDNA signals): (i) cyclic voltammetry (CV), square wave voltammetry (SWV), and electrochemical impedance spectroscopy (EIS) at the dsDNA/GCE biosensor, and (ii) differential pulse voltammetry (DPV) for measurements directly in solution at the bare GCE. The changes in dsDNA structure caused by structurally similar analytes were observed. The mutual interaction between derivatives of fluorene and dsDNA caused damaging effects of the test substances on the dsDNA structure in vitro.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
<a href="/en/project/GA20-01589S" target="_blank" >GA20-01589S: New strategies for improving sensing properties of novel electrode materials via their surface pretreatment or modification</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Electroanalytical Chemistry
ISSN
1572-6657
e-ISSN
1873-2569
Volume of the periodical
918
Issue of the periodical within the volume
August
Country of publishing house
CH - SWITZERLAND
Number of pages
11
Pages from-to
116430
UT code for WoS article
000817795100007
EID of the result in the Scopus database
2-s2.0-85131958610