Correlative Fluorescence and Scanning Electron Microscopy of Labelled Core Fucosylated Glycans Using Cryosections Mounted on Carbon-Patterned Glass Slides
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F15%3A10371536" target="_blank" >RIV/00216208:11310/15:10371536 - isvavai.cz</a>
Alternative codes found
RIV/60076658:12310/15:43890245 RIV/60077344:_____/15:00467725
Result on the web
<a href="http://dx.doi.org/10.1371/journal.pone.0145034" target="_blank" >http://dx.doi.org/10.1371/journal.pone.0145034</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1371/journal.pone.0145034" target="_blank" >10.1371/journal.pone.0145034</a>
Alternative languages
Result language
angličtina
Original language name
Correlative Fluorescence and Scanning Electron Microscopy of Labelled Core Fucosylated Glycans Using Cryosections Mounted on Carbon-Patterned Glass Slides
Original language description
The aim of the study is co-localization of N-glycans with fucose attached to N-acetylglucosamine in alpha 1,3 linkage, that belong to immunogenic carbohydrate epitopes in humans, and N-glycans with alpha 1,6-core fucose typical for mammalian type of N-linked glycosylation. Both glycan epitopes were labelled in cryosections of salivary glands isolated from the tick Ixodes ricinus. Salivary glands secrete during feeding many bioactive molecules and influence both successful feeding and transmission of tick-borne pathogens. For accurate and reliable localization of labelled glycans in both fluorescence and scanning electron microscopes, we used carbon imprints of finder or indexed EM grids on glass slides. We discuss if the topographical images can provide information about labelled structures, the working setting of the field-emission scanning electron microscope and the influence of the detector selection (a below-the-lens Autrata improved YAG detector of back-scattered electrons; in-lens and conventional Everhart-Thornley detectors of secondary electrons) on the imaging of gold nanoparticles, quantum dots and osmium-stained membranes.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30224 - Radiology, nuclear medicine and medical imaging
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
PLoS One
ISSN
1932-6203
e-ISSN
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Volume of the periodical
10
Issue of the periodical within the volume
12
Country of publishing house
US - UNITED STATES
Number of pages
11
Pages from-to
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UT code for WoS article
000367092300033
EID of the result in the Scopus database
2-s2.0-84956660074