Interaction study of methyl violet 2B with DNA and voltammetric determination of DNA in aqueous solutions

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F16%3A10297240" target="_blank" >RIV/00216208:11310/16:10297240 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1007/s00706-015-1590-9" target="_blank" >http://dx.doi.org/10.1007/s00706-015-1590-9</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00706-015-1590-9" target="_blank" >10.1007/s00706-015-1590-9</a>

Result language

angličtina

Original language name

Interaction study of methyl violet 2B with DNA and voltammetric determination of DNA in aqueous solutions

Original language description

Differential pulse voltammetry, cyclic voltammetry, and chronocoulometry at a hanging mercury drop electrode were used for the study of the interaction of methyl violet 2B (MV) with double-stranded DNA in 0.1 mol dm(-3) acetate buffer pH 4.0. The mechanism of the electrochemical reduction of MV was also investigated, using cyclic voltammetry at a hanging mercury drop electrode. UV-Vis spectrophotometry was used for the investigation of the interaction as well. The kinetic parameters of the reduction ofMV and the DNA-MV complex and the thermodynamic parameters of the formed DNA-MV complex were calculated. Moreover, the calibration dependence of the peak current of MV on the concentration of DNA in acetate buffer was constructed to be used for the indirect determination of DNA based on the decreasing peak current of MV with increasing concentration of DNA in the measured solution. [GRAPHICS] .

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CG - Electrochemistry

OECD FORD branch

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Project

<a href="/en/project/GBP206%2F12%2FG151" target="_blank" >GBP206/12/G151: Center of novel approaches to bioanalysis and molecular diagnostics</a><br>

Continuities

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Monatshefte für Chemie

ISSN

0026-9247

e-ISSN

—

Volume of the periodical

147

Issue of the periodical within the volume

1

Country of publishing house

AT - AUSTRIA

Number of pages

8

Pages from-to

119-126

UT code for WoS article

000367521400016

EID of the result in the Scopus database

2-s2.0-84952802369