Ethylenediaminetetraacetic Acid, Sodium Citrate, Heparin and Citrate Phosphate Dextrose-Adenine Anticoagulants Differentially Affect Cytokine mRNA Expression in Blood Leukocytes
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F16%3A10330202" target="_blank" >RIV/00216208:11310/16:10330202 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.7754/Clin.Lab.2016.151140" target="_blank" >http://dx.doi.org/10.7754/Clin.Lab.2016.151140</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.7754/Clin.Lab.2016.151140" target="_blank" >10.7754/Clin.Lab.2016.151140</a>
Alternative languages
Result language
angličtina
Original language name
Ethylenediaminetetraacetic Acid, Sodium Citrate, Heparin and Citrate Phosphate Dextrose-Adenine Anticoagulants Differentially Affect Cytokine mRNA Expression in Blood Leukocytes
Original language description
Background: The aim of this study is to determine whether and how the anticoagulant agents EDTA, sodium citrate, and heparin present in vacutainers and CPDA located in transfusion bags modify peripheral blood cell behavior. Methods: We compared the effect of four anticoagulants within an expression study quantifying mRNA by realtime PCR in 60 blood samples. Results: We observed a significant increase of TNF alpha and CCL2 mRNA expression in leukocytes placed in EDTA anticoagulant compared to leukocytes collected with citrate or heparin (p <= 0.5). Compared to blood cells influenced by one of the three anticoagulant agents present in vacuum tubes, cells from transfusion bags affected by CPDA displayed significantly lower levels of CCL2 and MYC mRNA, whereas levels of IL-10 and TNF alpha mRNA were much higher (p <= 0.5). Conclusions: The anticoagulant EDTA significantly increases proinflammatory cytokine mRNA in freshly isolated PBMC. Anticoagulant CPDA significantly alters levels of some of the tested mRNAs in comparison to blood cells drawn into one of the three anticoagulant agents present in vacutainers.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Clinical Laboratory
ISSN
1433-6510
e-ISSN
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Volume of the periodical
62
Issue of the periodical within the volume
7
Country of publishing house
DE - GERMANY
Number of pages
4
Pages from-to
1371-1374
UT code for WoS article
000385813300026
EID of the result in the Scopus database
2-s2.0-84981187663