Prolonged Morphine Treatment Alters Expression and Plasma Membrane Distribution of beta-Adrenergic Receptors and Some Other Components of Their Signaling System in Rat Cerebral Cortex
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F17%3A10368555" target="_blank" >RIV/00216208:11310/17:10368555 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1007/s12031-017-0987-9" target="_blank" >http://dx.doi.org/10.1007/s12031-017-0987-9</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s12031-017-0987-9" target="_blank" >10.1007/s12031-017-0987-9</a>
Alternative languages
Result language
angličtina
Original language name
Prolonged Morphine Treatment Alters Expression and Plasma Membrane Distribution of beta-Adrenergic Receptors and Some Other Components of Their Signaling System in Rat Cerebral Cortex
Original language description
beta-Adrenergic signaling plays an important role in regulating diverse brain functions and alterations in this signaling have been observed in different neuropathological conditions. In this study, we investigated the effect of a 10-day treatment with high doses of morphine (10 mg/kg per day) on major components and functional state of the beta-adrenergic receptor (beta-AR) signaling system in the rat cerebral cortex. beta-ARs were characterized by radioligand binding assays and amounts of various G protein subunits, adenylyl cyclase (AC) isoforms, G protein-coupled receptor kinases (GRKs), and beta-arrestin were examined by Western blot analysis. AC activity was determined as a measure of functionality of the signaling system. We also assessed the partitioning of selected signaling proteins between the lipid raft and non-raft fractions prepared from cerebrocortical plasma membranes. Morphine treatment resulted in a significant upregulation of beta-ARs, GRK3, and some AC isoforms (AC-I, -II, and -III). There was no change in quantity of G proteins and some other signaling molecules (AC-IV, AC-V/VI, GRK2, GRK5, GRK6, and beta-arrestin) compared with controls. Interestingly, morphine exposure caused a partial redistribution of beta-ARs, G(s)alpha, G(o)alpha, and GRK2 between lipid rafts and bulk plasma membranes. Spatial localization of other signaling molecules within the plasma membrane was not changed. Basal as well as fluoride- and forskolin-stimulated AC activities were not significantly different in membrane preparations from control and morphine-treated animals. However, AC activity stimulated by the beta-AR agonist isoprenaline was markedly increased. This is the first study to demonstrate lipid raft association of key components of the cortical beta-AR system and its sensitivity to morphine.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30105 - Physiology (including cytology)
Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Molecular Neuroscience
ISSN
0895-8696
e-ISSN
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Volume of the periodical
63
Issue of the periodical within the volume
3-4
Country of publishing house
US - UNITED STATES
Number of pages
13
Pages from-to
364-376
UT code for WoS article
000415983800011
EID of the result in the Scopus database
2-s2.0-85032393137