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Components of the G(s) signaling cascade exhibit distinct changes in mobility and membrane domain localization upon beta(2)-adrenergic receptor activation

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F20%3A43902459" target="_blank" >RIV/60076658:12310/20:43902459 - isvavai.cz</a>

  • Result on the web

    <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7333016/" target="_blank" >https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7333016/</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1111/tra.12724" target="_blank" >10.1111/tra.12724</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Components of the G(s) signaling cascade exhibit distinct changes in mobility and membrane domain localization upon beta(2)-adrenergic receptor activation

  • Original language description

    The G protein signaling cascade is a key player in cell signaling. Cascade activation leads to a redistribution of its members in various cellular compartments. These changes are likely related to the &quot;second wave&quot; of signaling from endosomes. Here, we set out to determine whether G(s) signaling cascade members expressed at very low levels exhibit altered mobility and localize in clathrin-coated structures (CCSs) or caveolae upon activation by beta(2)-adrenergic receptors (beta(2)AR). Activated beta(2)AR showed decreased mobility and sustained accumulation in CCSs but not in caveolae. Arrestin 3 translocated to the plasma membrane after beta(2)AR activation and showed very low mobility and pronounced accumulation in CCSs. In contrast, G alpha(s) and G gamma(2) exhibited a modest reduction in mobility but no detectable accumulation in or exclusion from CCSs or caveolae. The effector adenylyl cyclase 5 (AC5) showed a slight mobility increase upon beta(2)AR stimulation, no redistribution to CCSs, and weak activation-independent accumulation in caveolae. Our findings show an overall decrease in the mobility of most activated G(s) signaling cascade members and confirm that beta(2)AR and arrestin 3 accumulate in CCSs, while G alpha(s), G gamma(2) and AC5 can transiently enter CCSs and caveolae but do not accumulate in and are not excluded from these domains.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10601 - Cell biology

Result continuities

  • Project

    <a href="/en/project/GJ20-11563Y" target="_blank" >GJ20-11563Y: Allosteric communication in membrane protein complexes linked to conformational transitions on multiple timescales</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2020

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Traffic

  • ISSN

    1398-9219

  • e-ISSN

  • Volume of the periodical

    21

  • Issue of the periodical within the volume

    4

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    9

  • Pages from-to

    324-332

  • UT code for WoS article

    000518877400002

  • EID of the result in the Scopus database

    2-s2.0-85081303588