Characterization of pollen-expressed bZIP protein interactions and the role of ATbZIP18 in the male gametophyte
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F17%3A10373163" target="_blank" >RIV/00216208:11310/17:10373163 - isvavai.cz</a>
Alternative codes found
RIV/67985823:_____/17:00482646 RIV/61389030:_____/17:00476392 RIV/61388963:_____/17:00482646 RIV/00023752:_____/17:43919307
Result on the web
<a href="https://doi.org/10.1007/s00497-016-0295-5" target="_blank" >https://doi.org/10.1007/s00497-016-0295-5</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s00497-016-0295-5" target="_blank" >10.1007/s00497-016-0295-5</a>
Alternative languages
Result language
angličtina
Original language name
Characterization of pollen-expressed bZIP protein interactions and the role of ATbZIP18 in the male gametophyte
Original language description
Transcriptional control of gene expression represents an important mechanism guiding organisms through developmental processes and providing plasticity towards environmental stimuli. Because of their sessile nature, plants require effective gene regulation for rapid response to variation in environmental and developmental conditions. Transcription factors (TFs) provide such control ensuring correct gene expression in spatial and temporal manner. Our work reports the interaction network of six bZIP TFs expressed in Arabidopsis thaliana pollen and highlights the potential functional role for AtbZIP18 in pollen. AtbZIP18 was shown to interact with three other pollen-expressed bZIP TFs-AtbZIP34, AtbZIP52, and AtbZIP61 in yeast two-hybrid assays. AtbZIP18 transcripts are highly expressed in pollen, and at the subcellular level, an AtbZIP18-GFP fusion protein was located in the nucleus and cytoplasm/ER. To address the role of AtbZIP18 in the male gametophyte, we performed phenotypic analysis of a T-DNA knockout allele, which showed slightly reduced transmission through the male gametophyte. Some of the phenotype defects in atbzip18 pollen, although observed at low penetrance, were similar to those seen at higher frequency in the T-DNA knockout of the interacting partner, AtbZIP34. To gain deeper insight into the regulatory role of AtbZIP18, we analysed atbzip18/- pollen microarray data. Our results point towards a potential repressive role for AtbZIP18 and its functional redundancy with AtbZIP34 in pollen.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10611 - Plant sciences, botany
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Plant Reproduction
ISSN
2194-7953
e-ISSN
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Volume of the periodical
30
Issue of the periodical within the volume
1
Country of publishing house
DE - GERMANY
Number of pages
17
Pages from-to
1-17
UT code for WoS article
000396118900001
EID of the result in the Scopus database
2-s2.0-84997712923