Characterization of pollen-expressed bZIP protein interactions and the role of ATbZIP18 in the male gametophyte

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F17%3A00476392" target="_blank" >RIV/61389030:_____/17:00476392 - isvavai.cz</a>

Alternative codes found

RIV/67985823:_____/17:00482646 RIV/61388963:_____/17:00482646 RIV/00023752:_____/17:43919307 RIV/00216208:11310/17:10373163

Result on the web

<a href="http://dx.doi.org/10.1007/s00497-016-0295-5" target="_blank" >http://dx.doi.org/10.1007/s00497-016-0295-5</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00497-016-0295-5" target="_blank" >10.1007/s00497-016-0295-5</a>

Result language

angličtina

Original language name

Characterization of pollen-expressed bZIP protein interactions and the role of ATbZIP18 in the male gametophyte

Original language description

Transcriptional control of gene expression represents an important mechanism guiding organisms through developmental processes and providing plasticity towards environmental stimuli. Because of their sessile nature, plants require effective gene regulation for rapid response to variation in environmental and developmental conditions. Transcription factors (TFs) provide such control ensuring correct gene expression in spatial and temporal manner. Our work reports the interaction network of six bZIP TFs expressed in Arabidopsis thaliana pollen and highlights the potential functional role for AtbZIP18 in pollen. AtbZIP18 was shown to interact with three other pollen-expressed bZIP TFs-AtbZIP34, AtbZIP52, and AtbZIP61 in yeast two-hybrid assays. AtbZIP18 transcripts are highly expressed in pollen, and at the subcellular level, an AtbZIP18-GFP fusion protein was located in the nucleus and cytoplasm/ER. To address the role of AtbZIP18 in the male gametophyte, we performed phenotypic analysis of a T-DNA knockout allele, which showed slightly reduced transmission through the male gametophyte. Some of the phenotype defects in atbzip18 pollen, although observed at low penetrance, were similar to those seen at higher frequency in the T-DNA knockout of the interacting partner, AtbZIP34. To gain deeper insight into the regulatory role of AtbZIP18, we analysed atbzip18/- pollen microarray data. Our results point towards a potential repressive role for AtbZIP18 and its functional redundancy with AtbZIP34 in pollen.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10611 - Plant sciences, botany

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Plant Reproduction

ISSN

2194-7953

e-ISSN

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Volume of the periodical

30

Issue of the periodical within the volume

1

Country of publishing house

DE - GERMANY

Number of pages

17

Pages from-to

1-17

UT code for WoS article

000396118900001

EID of the result in the Scopus database

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