Biological characterization of a novel hybrid copolymer carrier system based on glycogen
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F18%3A10365838" target="_blank" >RIV/00216208:11310/18:10365838 - isvavai.cz</a>
Alternative codes found
RIV/67985823:_____/18:00484217 RIV/61389013:_____/18:00484217 RIV/00216208:11110/18:10365838 RIV/00023001:_____/18:00076899
Result on the web
<a href="https://link.springer.com/article/10.1007/s13346-017-0436-x" target="_blank" >https://link.springer.com/article/10.1007/s13346-017-0436-x</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s13346-017-0436-x" target="_blank" >10.1007/s13346-017-0436-x</a>
Alternative languages
Result language
angličtina
Original language name
Biological characterization of a novel hybrid copolymer carrier system based on glycogen
Original language description
The effective drug delivery systems for cancer treatment are currently on high demand. In this paper, biological behavior of the novel hybrid copolymers based on polysaccharide glycogen were characterized. The copolymers were modified by fluorescent dyes for flow cytometry, confocal microscopy, and in vivo fluorescence imaging. Moreover, the effect of oxazoline grafts on degradation rate was examined. Intracellular localization, cytotoxicity, and internalization route of the modified copolymers were examined on HepG2 cell line. Biodistribution of copolymers was addressed by in vivo fluorescence imaging in C57BL/6 mice. Our results indicate biocompatibility, biodegradability, and non-toxicity of the glycogen-based hybrid copolymers. Copolymers were endocyted into the cytoplasm, most probably via caveolae-mediated endocytosis. Higher content of oxazoline in polymers slowed down cellular uptake. No strong colocalization of the glycogen-based probe with lysosomes was observed; thus, it seems that the modified externally administered glycogen is degraded in the same way as an endogenous glycogen. In vivo experiment showed relatively fast biodistribution and biodegradation. In conclusion, this novel nanoprobe offers unique chemical and biological attributes for its use as a novel drug delivery system that might serve as an efficient carrier for cancer therapeutics with multimodal imaging properties.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10600 - Biological sciences
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Drug Delivery and Translational Research
ISSN
2190-393X
e-ISSN
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Volume of the periodical
8
Issue of the periodical within the volume
1
Country of publishing house
US - UNITED STATES
Number of pages
10
Pages from-to
73-82
UT code for WoS article
000428713100008
EID of the result in the Scopus database
2-s2.0-85040062084