FSCS Reveals the Complexity of Lipid Domain Dynamics in the Plasma Membrane of Live Cells
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F18%3A10388456" target="_blank" >RIV/00216208:11310/18:10388456 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.1016/j.bpj.2018.04.050" target="_blank" >https://doi.org/10.1016/j.bpj.2018.04.050</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.bpj.2018.04.050" target="_blank" >10.1016/j.bpj.2018.04.050</a>
Alternative languages
Result language
angličtina
Original language name
FSCS Reveals the Complexity of Lipid Domain Dynamics in the Plasma Membrane of Live Cells
Original language description
The coexistence of lipid domains with different degrees of lipid packing in the plasma membrane of mammalian cells has been postulated, but direct evidence has so far been challenging to obtain because of the small size and short lifetime of these domains in live cells. Here, we use fluorescence spectral correlation spectroscopy in conjunction with a probe sensitive to the membrane environment to quantify spectral fluctuations associated with dynamics of membrane domains in live cells. With this method, we show that membrane domains are present in live COS-7 cells and have a lifetime lower bound of 5.90 and 14.69 ms for the ordered and disordered phases, respectively. Comparisons to simulations indicate that the underlying mechanism of these fluctuations is complex but qualitatively described by a combination of dye diffusion between membrane domains as well as the motion of domains within the membrane.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10600 - Biological sciences
Result continuities
Project
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Continuities
O - Projekt operacniho programu
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Biophysical Journal
ISSN
0006-3495
e-ISSN
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Volume of the periodical
114
Issue of the periodical within the volume
12
Country of publishing house
US - UNITED STATES
Number of pages
10
Pages from-to
2855-2864
UT code for WoS article
000436471800012
EID of the result in the Scopus database
2-s2.0-85048497774