Lipid Driven Nanodomains in Giant Lipid Vesicles are Fluid and Disordered
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F17%3A00476382" target="_blank" >RIV/61388955:_____/17:00476382 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1038/s41598-017-05539-y" target="_blank" >http://dx.doi.org/10.1038/s41598-017-05539-y</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/s41598-017-05539-y" target="_blank" >10.1038/s41598-017-05539-y</a>
Alternative languages
Result language
angličtina
Original language name
Lipid Driven Nanodomains in Giant Lipid Vesicles are Fluid and Disordered
Original language description
It is a fundamental question in cell biology and biophysics whether sphingomyelin (SM)- and cholesteroln(Chol)- driven nanodomains exist in living cells and in model membranes. Biophysical studies on modelnmembranes revealed SM and Chol driven micrometer-sized liquid-ordered domains. Although thenexistence of such microdomains has not been proven for the plasma membrane, such lipid mixturesnhave been often used as a model system for ‘rafts’. On the other hand, recent super resolution andnsingle molecule results indicate that the plasma membrane might organize into nanocompartments.nHowever, due to the limited resolution of those techniques their unambiguous characterizationnis still missing. In this work, a novel combination of Förster resonance energy transfer and MontenCarlo simulations (MC-FRET) identifies directly 10 nm large nanodomains in liquid-disordered modelnmembranes composed of lipid mixtures containing SM and Chol. Combining MC-FRET with solidstatenwide-line and high resolution magic angle spinning NMR as well as with fluorescence correlationnspectroscopy we demonstrate that these nanodomains containing hundreds of lipid molecules are fluidnand disordered. In terms of their size, fluidity, order and lifetime these nanodomains may represent anrelevant model system for cellular membranes and are closely related to nanocompartments suggestednto exist in cellular membranes.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10403 - Physical chemistry
Result continuities
Project
<a href="/en/project/GA17-03160S" target="_blank" >GA17-03160S: The role of gangliosides in the development of neurodegenerative amyloidogenic diseases: Molecular insights by advanced fluorescence microscopy</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Scientific Reports
ISSN
2045-2322
e-ISSN
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Volume of the periodical
7
Issue of the periodical within the volume
JUL 2017
Country of publishing house
GB - UNITED KINGDOM
Number of pages
12
Pages from-to
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UT code for WoS article
000405464200097
EID of the result in the Scopus database
2-s2.0-85024403161