Heterogeneous Lipid Distributions in Membranes as Revealed by Electronic Energy Transfer
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F16%3A00457553" target="_blank" >RIV/61388955:_____/16:00457553 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1007/978-3-319-24609-3_7" target="_blank" >http://dx.doi.org/10.1007/978-3-319-24609-3_7</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/978-3-319-24609-3_7" target="_blank" >10.1007/978-3-319-24609-3_7</a>
Alternative languages
Result language
angličtina
Original language name
Heterogeneous Lipid Distributions in Membranes as Revealed by Electronic Energy Transfer
Original language description
The techniques achieving the highest resolution only can characterize/nmembrane heterogeneities on the lowest molecular level. When F€orster resonance/nenergy transfer (FRET) is combined with Monte-Carlo (MC) simulations and is/napplied to the measurement of nanodomain/pore sizes it reaches an unbeatable/nresolution of 2–50 nm. While other techniques start being less efficient at such/nshort distances FRET is most efficient in this region. Here, usefulness of/nMC-FRET is demonstrated on three different systems that contain heterogeneously/ndistributed lipids: a nanoscopically phase separated bilayer, a bilayer containing/npores and finally on bicelles consisting of highly curved and flat regions. Moreover,/nthis paper gives the reader information on how a FRET experiment should be/ndesigned to achieve the highest FRET resolution but also which experimental/nconditions should be avoided. The theory describing FRET between randomly/ndistributed donors and acceptors in a lipid bilayer is also described in this paper as/nwell as reasons are explained why for heterogeneous probe distribution MC simulations/nshould rather be used.
Czech name
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Czech description
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Classification
Type
C - Chapter in a specialist book
CEP classification
CF - Physical chemistry and theoretical chemistry
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GC14-03141J" target="_blank" >GC14-03141J: Exploring the structure function relationship of membrane-pore-forming FGF2 oligomers - a single molecule approach</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Book/collection name
Reviews in Fluorescence 2015
ISBN
978-3-319-24609-3
Number of pages of the result
17
Pages from-to
171-187
Number of pages of the book
367
Publisher name
Springer
Place of publication
New York
UT code for WoS chapter
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