The Analysis of In-Membrane Nanoscopic Aggregation of Lipids and Proteins by MC-FRET

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F23%3A00571347" target="_blank" >RIV/61388955:_____/23:00571347 - isvavai.cz</a>
Alternative codes found
RIV/61388963:_____/23:00571347 RIV/00216208:11320/22:10447432
Result on the web
<a href="http://dx.doi.org/10.1007/4243_2022_29" target="_blank" >http://dx.doi.org/10.1007/4243_2022_29</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/4243_2022_29" target="_blank" >10.1007/4243_2022_29</a>

Result language
angličtina
Original language name
The Analysis of In-Membrane Nanoscopic Aggregation of Lipids and Proteins by MC-FRET
Original language description
Many lipids and membrane proteins spontaneously co-cluster and oligomerize in cellular plasma membranes into larger (functional) units, whose detailed characterization requires high spatial resolution. In this contribution, we introduce a powerful spectroscopy/microscopy approach called MC-FRET developed for the analysis of in-membrane nanoscopic aggregation of lipids and proteins in biological membranes. The approach is based on Förster resonance energy transfer (FRET) occurring in membranes between fluorescently labelled lipids/proteins and subsequent analysis of data by Monte-Carlo simulations. The following applications of MC-FRET are presented here: (1) determination of lipid nanodomain sizes and their surface density, (2) characterization of inter-leaflet organization of lipid nanodomains and (3) the analysis of dimerization of in-membrane proteins.
Czech name
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Czech description
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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