Determination of short-chain fatty acids in feces by capillary electrophoresis with indirect UV-VIS detection
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F19%3A10399165" target="_blank" >RIV/00216208:11310/19:10399165 - isvavai.cz</a>
Result on the web
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=hTVOc7CvZy" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=hTVOc7CvZy</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1039/c9ay01202h" target="_blank" >10.1039/c9ay01202h</a>
Alternative languages
Result language
angličtina
Original language name
Determination of short-chain fatty acids in feces by capillary electrophoresis with indirect UV-VIS detection
Original language description
Fermentation of undigested carbohydrates and amino acids in the large intestine leads to the production of short-chain fatty acids (SCFAs). Increasing attention has been recently paid to investigating the effect of the presence of SCFAs in the mammalian large intestine on the colon health. Thus, clinical practice requires analytical methods capable of fast, reliable and inexpensive quantification of SCFAs. Capillary electrophoresis meets such requirements, and hence a capillary electrophoresis method was developed to quantify acetate, propionate, butyrate and isobutyrate in human and canine feces. Benzoic acid in the background electrolyte was selected as a suitable indirect probe for indirect UV-VIS detection. A considerable advantage of the proposed method is the determination of butyric acid as well as isobutyric acid in one analytical run. The separation of these isomers was achieved through addition of alpha-cyclodextrin to the background electrolyte. The optimized method offers quantification of acetate at concentrations above 10 mu M, and propionate, butyrate and isobutyrate at concentrations above 8 mu M with acceptable precision, accuracy and with no significant matrix effects.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Analytical Methods
ISSN
1759-9660
e-ISSN
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Volume of the periodical
11
Issue of the periodical within the volume
36
Country of publishing house
GB - UNITED KINGDOM
Number of pages
5
Pages from-to
4575-4579
UT code for WoS article
000487048000003
EID of the result in the Scopus database
2-s2.0-85072626461