Systematic evaluation of selected supercritical fluid chromatography diol- and diethylamine-based columns for application in hydrophilic interaction liquid chromatography
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F19%3A10403529" target="_blank" >RIV/00216208:11310/19:10403529 - isvavai.cz</a>
Result on the web
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=JL0fubJd2y" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=JL0fubJd2y</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/sscp.201900023" target="_blank" >10.1002/sscp.201900023</a>
Alternative languages
Result language
angličtina
Original language name
Systematic evaluation of selected supercritical fluid chromatography diol- and diethylamine-based columns for application in hydrophilic interaction liquid chromatography
Original language description
Hydrophilic interaction liquid chromatography has become a useful method for separation of polar and hydrophilic analytes, i.e. compounds that are difficult to separate in reversed-phase chromatography. The retention mechanism in hydrophilic interaction liquid chromatography is rather complex. Different types of interactions contribute to retention, depending on the properties of stationary phase, mobile phase and nature of analytes. Many columns for hydrophilic interaction liquid chromatography are available and the new stationary phases are still under development. In our study systematic examination to describe two novel columns, intended for supercritical fluid chromatography, as potential stationary phases for hydrophilic interaction liquid chromatography was performed. The supercritical fluid chromatography columns contained polar diethylamine ligand and diol ligand bonded to ethylene bridged hybrid particles. The retentivity and selectivity of the stationary phases were evaluated using a variety of test compounds. The impacts of acetonitrile content, buffer pH and concentration on the retention were investigated. Distinct mixed-mode behavior of the diethylamine column was observed; significant contribution of anion-exchange interaction to retention was confirmed. The DIOL phase has neutral character and retained analytes predominantly by hydrophilic interaction liquid chromatography mechanism.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
<a href="/en/project/GA16-05942S" target="_blank" >GA16-05942S: Gradient chromatofocusing and isoelectric focusing - tools for separation of proteins</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Separation Science Plus [online]
ISSN
2573-1815
e-ISSN
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Volume of the periodical
2
Issue of the periodical within the volume
3
Country of publishing house
GB - UNITED KINGDOM
Number of pages
8
Pages from-to
81-88
UT code for WoS article
000476952900002
EID of the result in the Scopus database
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