Effect of water on the retention on diol and amide columns in hydrophilic interaction liquid chromatography
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F17%3A39911192" target="_blank" >RIV/00216275:25310/17:39911192 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1002/jssc.201601044" target="_blank" >http://dx.doi.org/10.1002/jssc.201601044</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/jssc.201601044" target="_blank" >10.1002/jssc.201601044</a>
Alternative languages
Result language
angličtina
Original language name
Effect of water on the retention on diol and amide columns in hydrophilic interaction liquid chromatography
Original language description
The amount of water adsorbed on polar columns plays important role in hydrophilic interaction liquid chromatography. It may strongly differ for the individual types of polar columns used in this separation mode. We measured adsorption isotherms of water on an amide and three diol-bonded stationary phases that differ in the chemistry of the bonded ligands and properties of the silica gel support. We studied the effects of the adsorbed water on the retention of aromatic carboxylic acids, flavonoids, benzoic acid derivatives, nucleic bases, and nucleosides in aqueous-acetonitrile mobile phases over the full composition range. The graphs of the retention factors versus the volume fraction of water in mobile phase show "U-profile" characteristic of a dual hydrophilic interaction-reversed phase retention mechanism. The minimum on the graph that marks the changing retention mechanism depends on the amount of adsorbed water. The linear solvation energy relationship model suggests that the retention in the hydrophilic interaction liquid chromatography mode is controlled mainly by proton-donor interactions in the stationary phase, depending on the column type. Finally, the accuracy of hydrophilic interaction liquid chromatography gradient prediction improves for columns that show a high water adsorption.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Separation Science
ISSN
1615-9306
e-ISSN
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Volume of the periodical
40
Issue of the periodical within the volume
7
Country of publishing house
DE - GERMANY
Number of pages
15
Pages from-to
1434-1448
UT code for WoS article
000399782900001
EID of the result in the Scopus database
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