Determination of low-molecular-mass heparin using affinity capillary electrophoresis

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F19%3A10409584" target="_blank" >RIV/00216208:11310/19:10409584 - isvavai.cz</a>
Result on the web
<a href="http://web.natur.cuni.cz/analchem/isc/isc15.pdf" target="_blank" >http://web.natur.cuni.cz/analchem/isc/isc15.pdf</a>
DOI - Digital Object Identifier
—

Result language
angličtina
Original language name
Determination of low-molecular-mass heparin using affinity capillary electrophoresis
Original language description
This work is dedicated to determination of low-molecular-mass heparin, namely Fraxiparine, using affinity capillary electrophoresis. Fraxiparine was detected indirectly by using tetraarginine, which forms a stable complex with Fraxiparine. The developed method used 50 μm i.d. fused silica capillary (length was optimized). Phosphoric acid (9 mM) with addition of 0.1% w/v hydroxyethylcellulose was used as a background electrolyte. The samples were injected hydrodynamically into the capillary by a pressure of 5 kPa. After that, voltage was applied for a certain period of time. During this time the zones migrate through each other, which enables formation of the complex. Fraxiparine was determined from the amount of remaining free tetraarginine. The injection time of Fraxiparine was subject to optimization. After the optimization, different experiments were performed for the determination of Fraxiparine in blood plasma. Acetonitrile, acetone and trifluoroacetic acid were tested for deproteination of the samples.
Czech name
—
Czech description
—

Project
—
Continuities
S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
Proceedings of the 15th International Students Conference "Modern Analytical Chemistry"
ISBN
978-80-7444-068-7
ISSN
—
e-ISSN
—
Number of pages
6
Pages from-to
39-44
Publisher name
Univerzita Karlova, Přírodovědecká fakulta
Place of publication
Praha
Event location
Prague
Event date
Sep 19, 2019
Type of event by nationality
EUR - Evropská akce
UT code for WoS article
000576787700006

Similar results(10)