Structural studies of PML nuclear bodies in polyomavirus infected cells
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F19%3A10458443" target="_blank" >RIV/00216208:11310/19:10458443 - isvavai.cz</a>
Result on the web
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=Y.s4fD6uty" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=Y.s4fD6uty</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.7124/bc.0009F4" target="_blank" >10.7124/bc.0009F4</a>
Alternative languages
Result language
angličtina
Original language name
Structural studies of PML nuclear bodies in polyomavirus infected cells
Original language description
ProMyelocytic Leukemia Nuclear Bodies (PML NBs) are distinct dynamic nuclear substructures (approx. 1 micron in diameter) implicated in different physiological and pathological cellular processes, including virus infection. While large viruses, e.g. herpesvi-ruses cause their disruption, smaller DNA viruses, as papilloma-or polyomaviruses, realize parts of the reproduction cycle in their close proximity. Previously, we found that Mouse polyomavirus (MPyV) infection causes multiplication and enlargement of PML NBs. During late phases of infection, the integrity and morphology of PML NBs are visibly altered. In addition, we observed the accumulation of MPyV virions around and inside of PML NBs. The aims of our research are: 1. To find whether replication of MPyV genomes itself or rather assembly of virions is responsible for altering the integrity and morphology of PML NBs. 2. To reveal the process of multiplication of PML NBs in infected cells. 3. To visualize the interaction of viral structural and regulatory proteins with PML NBs. We found that replication of mutated MPyV, capable of genome replication and production of all regulatory proteins, is sufficient to alter the morphology of PML NBs, althought it lacks ability to produce structural proteins. Live cell microscopy revealed that in infected cells, PML NBs are highly dynamic structures that assemble from soluble PML NBs' proteins as well as by fusion or fission of pre-existing nuclear bodies. Using structured illumination microscopy (SIM) and stochastic optical reconstruction microscopy (STORM), we observed the major structural protein of MPyV VP1,-to be located inside PML NBs, while the regulatory large T antigen ( bound to replicating MPyV genomes) was located by the surface of PML NBs.
Czech name
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Czech description
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Classification
Type
J<sub>SC</sub> - Article in a specialist periodical, which is included in the SCOPUS database
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Biopolymers and Cell
ISSN
0233-7657
e-ISSN
1993-6842
Volume of the periodical
35
Issue of the periodical within the volume
3
Country of publishing house
UA - UKRAINE
Number of pages
1
Pages from-to
229
UT code for WoS article
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EID of the result in the Scopus database
2-s2.0-85077218398