Co-localization of Interleukin-1 alpha and Annexin A2 at the plasma membrane in response to oxidative stress

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F20%3A10414160" target="_blank" >RIV/00216208:11310/20:10414160 - isvavai.cz</a>

Result on the web

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=.VfEFZ4XCG" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=.VfEFZ4XCG</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.cyto.2020.155141" target="_blank" >10.1016/j.cyto.2020.155141</a>

Result language

angličtina

Original language name

Co-localization of Interleukin-1 alpha and Annexin A2 at the plasma membrane in response to oxidative stress

Original language description

Interleukin-1 alpha (IL-1 alpha) and Annexin A2 (AnxA2) are pleiotropic molecules with both intracellular and extracellular roles. They share several characteristics including unconventional secretion aided by S100 proteins, anchoring of the externalized proteins at the outer surface of the plasma membrane and response to oxidative stress. Although IL-1 alpha and AnxA2 have been implicated in a variety of biological processes, including cancer, little is known about the mechanisms of their cellular release. In the present study, employing the non-cancerous breast epithelial MCF10A cells, we demonstrate that IL-1 alpha and AnxA2 establish a close association in response to oxidative stress. Stress conditions lead to translocation of both proteins towards lamellipodia rich in vimentin and association of full-length IL-1 alpha and Tyr23 phosphorylated AnxA2 with the plasma membrane at peripheral sites depleted of F-actin. Notably, membrane-associated IL-1 alpha and AnxA2 preferentially localize to the outer edges of the MCF10A cell islands, suggesting that the two proteins participate in the communication of these epithelial cells with their neighboring cells. Similarly, in U2OS osteosarcoma cell line both endogenous IL-1 alpha and transiently produced IL-1 alpha/EGFP associate with the plasma membrane. While benign MFC10A cells present membrane-associated IL-1 alpha and AnxA2 at the edges of their cell islands, the aggressive cancerous U2OS cells communicate in such manner also with distant cells.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10600 - Biological sciences

Project

<a href="/en/project/LM2015062" target="_blank" >LM2015062: National Infrastructure for Biological and Medical Imaging</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Cytokine

ISSN

1043-4666

e-ISSN

—

Volume of the periodical

133

Issue of the periodical within the volume

September

Country of publishing house

GB - UNITED KINGDOM

Number of pages

13

Pages from-to

155141

UT code for WoS article

000554002300019

EID of the result in the Scopus database

2-s2.0-85086946959