Development of a simple high-throughput assay for directed evolution of enantioselective sulfoxide reductases

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F20%3A10414595" target="_blank" >RIV/00216208:11310/20:10414595 - isvavai.cz</a>
Result on the web
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=d1jsbUyXhb" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=d1jsbUyXhb</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1039/d0cc01660h" target="_blank" >10.1039/d0cc01660h</a>

Result language
angličtina
Original language name
Development of a simple high-throughput assay for directed evolution of enantioselective sulfoxide reductases
Original language description
We report on the development of high-throughput fluorogenic assay that can streamline directed evolution of enantioselective sulfoxide reductases. As a model, methionine sulfoxide reductase A (MsrA) has been evolved to expand its limited substrate scope. The resulting mutant MsrA can resolve a range of new challenging racemic sulfoxides with high efficiency including the pharmaceutically relevant albendazole sulfoxide. The simplicity and the level of throughput make this method also suitable for the screening of metagenomic libraries in future for the discovery of new enzymes with similar reactivities.
Czech name
—
Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10401 - Organic chemistry

Project
<a href="/en/project/GJ17-25897Y" target="_blank" >GJ17-25897Y: Development of Fluorescent Probes for Methionine Sulfoxide Reductase and Beyond</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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