Complexation of DNA with QPDMAEMA-b-PLMA-b-POEGMA Cationic Triblock Terpolymer Micelles
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F20%3A10419519" target="_blank" >RIV/00216208:11310/20:10419519 - isvavai.cz</a>
Result on the web
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=0isX1y.MWX" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=0isX1y.MWX</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1021/acs.macromol.0c00388" target="_blank" >10.1021/acs.macromol.0c00388</a>
Alternative languages
Result language
angličtina
Original language name
Complexation of DNA with QPDMAEMA-b-PLMA-b-POEGMA Cationic Triblock Terpolymer Micelles
Original language description
In this work, we investigate the formation and structure of complexes between quaternized poly(2-(dimethylamino)ethyl methacrylate-b-lauryl methacrylate-b-(oligo ethylene glycol) methacrylate) (QPDMAEMA-b-PLMA-b-POEGMA) amphiphilic triblock terpolymer micelles, with different compositions, and DNAs of different lengths. In aqueous solutions, the terpolymers self-assemble in spherical micelles comprising hydrophobic PLMA cores and mixed QPDMAEMA/POEGMA coronas. Terpolymer/DNA micelleplexes were prepared in a wide range of amine over phosphate group ratios (N/P), and linear DNA of different lengths (2000 base pairs and 113 base pairs) was utilized. The complexation ability of the polymeric micelles with DNA was studied by UV-vis spectroscopy and ethidium bromide quenching assays using fluorescence spectroscopy. The thermodynamic interactions that take place during complexation were investigated by isothermal titration calorimetry, whereas the structural characteristics of the micelleplexes were determined by lights-cattering techniques (dynamic, static, and electrophoretic) and cryogenic transmission electron microscopy (cryo-TEM) imaging. All terpolymer micelles effectively complex DNAs, forming micelleplexes, with their size, surface charge, and structure depending on the ratio of the components, length of the DNA, and the chemical structure of the terpolymers. Cryo-TEM revealed the clustering of terpolymer micelles due to complexation with the clusters having different topologies influenced remarkably by the length of the DNA utilized.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10404 - Polymer science
Result continuities
Project
<a href="/en/project/LTAIN19078" target="_blank" >LTAIN19078: Stimuli-Responsive Block Copolymer Based Nanostructured Single-/Multi-component Micelles: Materials and Applications</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Macromolecules
ISSN
0024-9297
e-ISSN
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Volume of the periodical
53
Issue of the periodical within the volume
14
Country of publishing house
US - UNITED STATES
Number of pages
9
Pages from-to
5747-5755
UT code for WoS article
000557761800010
EID of the result in the Scopus database
2-s2.0-85088022590