The dynamics of band (peak) shape development in capillary zone electrophoresis in the case of two co-migrating analytes: The displacement and the tag-along effects
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F20%3A10419869" target="_blank" >RIV/00216208:11310/20:10419869 - isvavai.cz</a>
Result on the web
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=DmY5kxTlJN" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=DmY5kxTlJN</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/elps.201900225" target="_blank" >10.1002/elps.201900225</a>
Alternative languages
Result language
angličtina
Original language name
The dynamics of band (peak) shape development in capillary zone electrophoresis in the case of two co-migrating analytes: The displacement and the tag-along effects
Original language description
Peak shapes in electrophoresis are often distorted from the ideal Gaussian shape due to disturbing phenomena, of which the most important is electromigration dispersion. For fully dissociated analytes, there is a tight analogy between nonlinear models describing a separation process in chromatography and electrophoresis. When the velocity of the separated analyte depends on the concentration of the co-analyte, the consequence is a mutual influence of the analytes couples, which distorts both analyte zones. In this paper, we introduce a nonlinear model of electromigration for the analysis of two co-migrating fully dissociated analytes. In the initial stages of separation, they influence each other, which causes much more complicated peak shapes. The analysis has revealed that the two most important phenomena-the displacement and the tag-along effects-are common both for nonlinear chromatography and electrophoresis, though their description is partly based on rather different phenomena. The comparison between the nonlinear model of electromigration we describe and the numerical computer solution of the original continuity equations has proven an almost perfect agreement. The predicted features in peak shapes in initial stages of separation have been fully confirmed by the experiments.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
<a href="/en/project/GA18-11776S" target="_blank" >GA18-11776S: Advanced theoretical and software tools for electrophoresis</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Electrophoresis
ISSN
0173-0835
e-ISSN
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Volume of the periodical
41
Issue of the periodical within the volume
7-8
Country of publishing house
DE - GERMANY
Number of pages
12
Pages from-to
481-492
UT code for WoS article
000491680700001
EID of the result in the Scopus database
2-s2.0-85074589886