A comparative study of covalent glucose oxidase and laccase immobilization techniques at powdered supports for biosensors fabrication

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F20%3A10420315" target="_blank" >RIV/00216208:11310/20:10420315 - isvavai.cz</a>

Alternative codes found

RIV/61388955:_____/20:00566826

Result on the web

<a href="http://web.natur.cuni.cz/analchem/isc/isc16.pdf" target="_blank" >http://web.natur.cuni.cz/analchem/isc/isc16.pdf</a>

DOI - Digital Object Identifier

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Result language

angličtina

Original language name

A comparative study of covalent glucose oxidase and laccase immobilization techniques at powdered supports for biosensors fabrication

Original language description

In order to develop the optimal strategy and to deepen the knowledge in the field of enzyme immobilization, three different techniques of covalent binding for two enzymes (glucose oxidase and laccase) at powdered surfaces were compared. Immobilization protocol was optimized by changing supports (two mesoporous silica powders (SBA-15, MCM-41) and a cellulose powder), the functionalized groups introduced at support surfaces (-NH2 and -COOH), and the methods of activation (glutaraldehyde and carbodiimide). Amino and carboxyl functionalized mesoporous silica and cellulose powders were prepared by silanization using (3-aminopropyl)triethoxysilane and carboxyethylsilanetriol, respectively. It was found that coupling of both enzymes by their -NH2 groups through glutaraldehyde to -NH2 functionalized supports, in particular SBA15-NH2 and cellulose-NH2 for glucose oxidase, MCM41-NH2 for laccase, showed the highest activity and the best stability.

Czech name

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Czech description

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Type

D - Article in proceedings

CEP classification

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OECD FORD branch

10406 - Analytical chemistry

Project

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Continuities

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

Proceedings of the 16th International Students Conference &quot;Modern Analytical Chemistry&quot;

ISBN

978-80-7444-079-3

ISSN

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e-ISSN

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Number of pages

6

Pages from-to

25-30

Publisher name

Univerzita Karlova, Přírodovědecká fakulta

Place of publication

Praha

Event location

Prague

Event date

Sep 17, 2020

Type of event by nationality

EUR - Evropská akce

UT code for WoS article

000623101400005