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Comparison of the Covalent Laccase Immobilization at Amino- and Carboxyl-functionalized Mesoporous Silica, Glassy Carbon, and Graphite Powders using Different Coupling Agents for Optimal Biosensor Preparation

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F21%3A10442066" target="_blank" >RIV/00216208:11310/21:10442066 - isvavai.cz</a>

  • Alternative codes found

    RIV/61388955:_____/21:00549133

  • Result on the web

    <a href="https://www.bestservis.eu/images/file/Sbornik_metody21.pdf" target="_blank" >https://www.bestservis.eu/images/file/Sbornik_metody21.pdf</a>

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Comparison of the Covalent Laccase Immobilization at Amino- and Carboxyl-functionalized Mesoporous Silica, Glassy Carbon, and Graphite Powders using Different Coupling Agents for Optimal Biosensor Preparation

  • Original language description

    In order to find the most suitable immobilization protocol, a comparison of three strategies based on the application of -NH2 and -COOH functionalized supports with the different activation agents (glutaraldehyde and carbodiimide) have been conducted for the covalent enzyme (laccase) attachment. Two kinds of the supports, namely mesoporous silica (SBA-15, MCM-41) and carbonaceous (glassy carbon, graphite) powders, have been used. It was found that a biosensor consisted of tubular detector of silver solid amalgam as a working electrode and the enzymatic mini-reactor with laccase covalently attached to glutaraldehyde activated -NH2 functionalized MCM-41 shows the best results regarding sensitivity and stability for dopamine detection.

  • Czech name

  • Czech description

Classification

  • Type

    D - Article in proceedings

  • CEP classification

  • OECD FORD branch

    10406 - Analytical chemistry

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2021

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Article name in the collection

    XL. Moderní Elektrochemické Metody: Sborník přednášek

  • ISBN

    978-80-905221-8-3

  • ISSN

  • e-ISSN

  • Number of pages

    5

  • Pages from-to

    248-252

  • Publisher name

    Lenka Srsenová - BEST Servis, Ústí nad Labem

  • Place of publication

    Ústí nad Labem

  • Event location

    Jetrichovice, Česká republika

  • Event date

    Nov 8, 2021

  • Type of event by nationality

    EUR - Evropská akce

  • UT code for WoS article