Influence of different covalent immobilization protocols on electroanalytical performance of laccase-based biosensors
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F22%3A00560207" target="_blank" >RIV/61388955:_____/22:00560207 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/22:10452222
Result on the web
<a href="https://hdl.handle.net/11104/0333217" target="_blank" >https://hdl.handle.net/11104/0333217</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.bioelechem.2022.108223" target="_blank" >10.1016/j.bioelechem.2022.108223</a>
Alternative languages
Result language
angličtina
Original language name
Influence of different covalent immobilization protocols on electroanalytical performance of laccase-based biosensors
Original language description
This paper presents a simple and effective flow-through electrochemical biosensor, consisting of Trametes versicolor laccase (Lac)-based mini-reactor and a tubular detector of silver solid amalgam (TD-AgSA), capable of rapid and selective detection of phenolic compounds. Amperometric detection relies on the reduction of the quinone molecule (formed during the enzymatic reaction in a mini-reactor) on TD-AgSA at –50 mV vs SCE. Since different enzyme immobilization techniques may contribute to differing biosensor performances, four covalent strategies for Lac attachment were compared: (i) through glutaraldehyde to supports −NH2, (ii) via disuccinimidyl suberate to supports −NH2, (iii) using EDC/NHS for Lac coupling by its −COOH groups to supports −NH2, and (iv) using EDC/NHS to supports −COOH. Additionally, five supports (mesoporous silica (SBA-15, MCM-41), cellulose, carbon-based (glassy carbon, graphite) powders) were investigated. It was found that different methods of immobilization, as well as different types of supports, significantly affect the amount of immobilized Lac and, in turn, the analytical characteristics of the obtained biosensors. Thus, TD-AgSA with enzymatic mini-reactor based on Lac covalently attached via glutaraldehyde to aminated MCM-41 proved to be the most promising biosensor, providing the best detection limit (18.3 μmol L–1) and the long-term stability (47.9 % of the initial response/4 months/100 measurements).
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10405 - Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)
Result continuities
Project
<a href="/en/project/GA20-01589S" target="_blank" >GA20-01589S: New strategies for improving sensing properties of novel electrode materials via their surface pretreatment or modification</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Bioelectrochemistry
ISSN
1567-5394
e-ISSN
1878-562X
Volume of the periodical
148
Issue of the periodical within the volume
DEC 2022
Country of publishing house
CH - SWITZERLAND
Number of pages
11
Pages from-to
108223
UT code for WoS article
000848663600006
EID of the result in the Scopus database
2-s2.0-85135961559